This glass insert is from the Clue slot machine which can be found in casinos in Las Vegas, Nevada. The image is of the six suspects, each holding a weapon, and the word "Clue" and "Winning Solutions". The image is very vibrant when lit from behind.
The condition of the glass is fair. There is a small chip in the lower left corner. Also, a scratch through the word Clue can be seen when lit from behind.
The Clue Video Slot machine is made by Mikohn: www.mikohn.com